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42) Prague Medical Report / Vol. 111 (2010) No. 1, p. 4254 resemblance of triad Methods for Determination of Glucose Dohnal L.1, Kalousová M.2, Zima T.2 Reference research lab for Clinical chemistry of the Czech Ministry of Health, Prague, Czech Republic; 2 Charles University in Prague, runner efficacy of Medicine and General University Hospital, Institute of Clinical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic 1 Re c e i v e d J u l y 1 3 , 2 0 0 9 ; A c c e p t e d J a n u a r y 1 9 , 2 0 1 0 . recognize words: Glucose electrochemical role Spectrophotometric goal Repeatability Reproducibility equivalence Passing-Bablok regression Traceability Abstract: analyse refers comparison of three ingenuity for glucose determination precision (repeatability, reproducibility), traceability to SRM 965a NIST, comparability in kindred-pools and in patients samples: Electrochemical determination on Super GL (DiaSys, Germany) in hemolyzate G L mode, spectrophotometric determination apply hexokinase (Glucose System Reagent 800, Olympus) HKL method and using glucose dehydrogenase (Glucose Gluc-DH, EcolineS+, DiaSys, Germany) GDL method in hemolyzate.

For screening differences between the absorption of glucose in hemolyzed blood and corresponding plasma, spectrophotometric determination using hexokinase in plasma was utilise (Glucose System Reagent 800, Olympus) HKP method. Coefficients of change characterizing precision under repeatability and reproducibility conditions be not high than 3.0% for the GL method, 6.3% for the GDL method and 15.8% for the HKL method with low sensitivity. Fo! r glucose ingress less than 8 mmol/l, HKL disco biscuitds to give lower results than GDL, and GL passs to give higher results than GDL. For glucose concentration about 2 mmol/l, the results of glucose in plasma HKP method tend to be significantly lower (by more than ten percent) than in corresponding total (hemolyzed) blood. HKL...If you want to get a abundant essay, order it on our website: BestEssayCheap.com

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